Cyclophilin A Controls Salmonella Internalization Levels and is Present at E. coli Actin-Rich Pedestals
Back to previous page
| Authors: | Dhanda, AS; Warren, KE; Chiu, RH; Guttman, JA |
| Year: | 2018 |
| Journal: | Anat. Rec. 301 Article Link (DOI) PubMed |
| Title: | Cyclophilin A Controls Salmonella Internalization Levels and is Present at E. coli Actin-Rich Pedestals |
| Abstract: | Salmonella enterica serovar Typhimurium (S. Typhimurium), enteropathogenic Escherichia coli (EPEC) and enterohemorrhagic E. coli (EHEC) commandeer the actin cytoskeleton of their host cells as a crucial step in their infectious processes. These pathogens depend on the injection of their own effectors directly into target host cells in order to usurp cellular signaling pathways that lead to morphological actin rearrangements in those cells. Here we show that the PPIase Cyclophilin A (CypA) is a novel component of S. Typhimurium-induced membrane ruffles and functions to restrict bacterial invasion levels, as in cells depleted of CypA, bacterial loads increase. We also demonstrate that CypA requires the EPEC effector Tir as well as pedestal formation for its recruitment to bacterial attachment sites and that its presence at pedestals also holds during EHEC infections. Finally, we demonstrate that CypA is found at lamellipodia; actin-rich structures at the leading edge of motile cells. Our findings further establish CypA as a component of dynamic actin-rich structures formed during bacterial infections and within cells in general. (C) 2018 Wiley Periodicals, Inc. |
Please send suggestions for improving this publication database to
sass-support@sfu.ca.
Departmental members may update their publication list.