Estimating the Bioconcentration Factors of Hydrophobic Organic Compounds from Biotransformation Rates Using Rainbow Trout Hepatocytes
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| Authors: | Trowell, JJ; Gobas, FAPC; Moore, MM; Kennedy, CJ |
| Year: | 2018 |
| Journal: | Arch. Environ. Contam. Toxicol. 75 Article Link (DOI) PubMed |
| Title: | Estimating the Bioconcentration Factors of Hydrophobic Organic Compounds from Biotransformation Rates Using Rainbow Trout Hepatocytes |
| Abstract: | Determining the biotransformation potential of commercial chemicals is critical for estimating their persistence in the aquatic environment. In vitro systems are becoming increasingly important as screening methods for assessing the potential for chemical metabolism. Depletion rate constants (k (d)) for several organic chemicals with high octanol-water partition coefficient (K (ow)) values (9-methylanthracene, benzo(a)pyrene, chrysene, and PCB-153) in rainbow trout hepatocytes were determined to estimate biotransformation rate constants (k (MET)) that were used in fish bioconcentration factor (BCF) models. Benzo[a]pyrene was rapidly biotransformed when incubated singly; however, its depletion rate constant (k (d)) declined 79% in a mixture of all four chemicals. Chrysene also exhibited significant biotransformation and its depletion rate constant declined by 50% in the mixture incubation. These data indicate that biotransformation rates determined using single chemicals may overestimate metabolism in environments containing chemical mixtures. Incubations with varying cell concentrations were used to determine whether cell concentration affected k (d) estimates. No statistically significant change in depletion rate constants were seen, possibly due to an increase in nonspecific binding of hydrophobic chemicals as cell density increased, decreasing overall biotransformation. A new model was used to estimate BCFs from k (MET) values calculated from empirically derived k (d) values. The inclusion of k (MET) in models resulted in significantly lower BCF values (compared k (MET) = 0). Modelled BCF values were consistent with empirically derived BCF values from the literature. |
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