Formation of membrane ridges and scallops by the F-BAR protein Nervous Wreck
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| Authors: | Becalska, AN; Kelley, CF; Berciu, C; Stanishneva-Konovalova, TB; Fu, XF; Wang, SY; Sokolova, OS; Nicastro, D; Rodal, AA |
| Year: | 2013 |
| Journal: | Mol. Biol. Cell 24: 2406-2418 Article Link (DOI) PubMed |
| Title: | Formation of membrane ridges and scallops by the F-BAR protein Nervous Wreck |
| Abstract: | Eukaryotic cells are defined by extensive intracellular compartmentalization, which requires dynamic membrane remodeling. FER/Cip4 homology-Bin/amphiphysin/Rvs (F-BAR) domain family proteins form crescent-shaped dimers, which can bend membranes into buds and tubules of defined geometry and lipid composition. However, these proteins exhibit an unexplained wide diversity of membrane-deforming activities in vitro and functions in vivo. We find that the F-BAR domain of the neuronal protein Nervous Wreck (Nwk) has a novel higher-order structure and membrane-deforming activity that distinguishes it from previously described F-BAR proteins. The Nwk F-BAR domain assembles into zigzags, creating ridges and periodic scallops on membranes in vitro. This activity depends on structural determinants at the tips of the F-BAR dimer and on electrostatic interactions of the membrane with the F-BAR concave surface. In cells, Nwk-induced scallops can be extended by cytoskeletal forces to produce protrusions at the plasma membrane. Our results define a new F-BAR membrane-deforming activity and illustrate a molecular mechanism by which positively curved F-BAR domains can produce a variety of membrane curvatures. These findings expand the repertoire of F-BAR domain mediated membrane deformation and suggest that unique modes of higher-order assembly can define how these proteins sculpt the membrane. |
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