Thermal modulation of the toxicokinetics of benzo[a]pyrene in isolated hepatocytes of sablefish (Anoplopoma fimbria), black rockfish (Sebastes melanops), and chub mackerel (Scomber japonicus)
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| Authors: | Johnston, BD; Alexander, G; Kennedy, CJ |
| Year: | 1999 |
| Journal: | Comparative Biochemistry and Physiology C-Pharmacology Toxicology & Endocrinology 124: 157-164 |
| Title: | Thermal modulation of the toxicokinetics of benzo[a]pyrene in isolated hepatocytes of sablefish (Anoplopoma fimbria), black rockfish (Sebastes melanops), and chub mackerel (Scomber japonicus) |
| Abstract: | Hepatocytes from sablefish (Anoplapoma fimbria), black rockfish (Sebastes melanops) and chub mackerel (Scomber japonicus) were isolated from 11 degrees C acclimated animals. The uptake, metabolism, and excretion of benzo[a]pyrene (B[a]P) in hepatocytes was measured at 6, 11 and 19 degrees C. Chub mackerel hepatocyte uptake rates were significantly lower (0.012 +/- 0.003 mu g/s per g cell) at 11 degrees C than black rockfish (0.028 +/- 0.009 mu g/s per g cell) or sablefish (0.032 +/- 0.012 mu g/s per g cell) hepatocytes at all temperatures. Hepatocytes metabolized B[a]P to phase I (1-8%) and phase II (92-99%) metabolites. Accumulation of phase II metabolites was lower in chub mackerel hepatocytes (0.016 +/- 0.004 mu g/h per g cell), than black rockfish (0.052 +/- 0.012 mu g/h per g cell), or sablefish hepatocytes (0.060 +/- 0.015 mu g/h per g cell). Phase II metabolite accumulation increased greatest with temperature in chub mackerel hepatocytes (Q(10) = 1.94 +/- 0.30), followed by sablefish (Q(10) = 1.65 +/- 0.30), and rockfish (Q(10) = 1.38 +/- 0.30). Sablefish hepatocytes had higher excretion rates of phase II metabolites (0.010 +/- 0.0023 mu g/h per g cell), than mackerel (0.0046 +/- 0.0009 mu g/h per g cell) or rockfish hepatocytes (0.0029 +/- 0.0008 mu g/h per g cell). Phase II metabolite excretion rates increased with temperature only in sablefish hepatocytes (Q(10) = 1.67 +/- 0.76). These differences in toxicokinetics may indicate distinct consequences for various species exposed to xenobiotics. (C) 1999 Elsevier Science Inc. All rights reserved. |
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