Invasive and adherent bacterial pathogens co-Opt host clathrin for infection.

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Authors: Veiga E, Guttman JA, Bonazzi M, Boucrot E, Toledo-Arana A, Lin AE, Enninga J, Pizarro-Cerd√° J, Finlay BB, Kirchhausen T, Cossart P.
Year: 2007
Journal: Cell Host & Microbe 2(5):340-51.    Website    Article Link (DOI)
Title: Invasive and adherent bacterial pathogens co-Opt host clathrin for infection.
Abstract: Infection by the bacterium Listeria monocytogenes depends on host cell clathrin. To determine whether this requirement is widespread, we analyzed infection models using diverse bacteria. We demonstrated that bacteria that enter cells following binding to cellular receptors (termed zippering bacteria) invade ina clathrin-dependent manner. In contrast, bacteria that inject effector proteins into host cells in order to gain entry (termed triggering bacteria) invade in a clathrin-independent manner. Strikingly, enteropathogenic Escherichia coli (EPEC) required clathrin to form actin-rich pedestals in host cells beneath adhering bacteria, even though this pathogen remains extracellular. Furthermore, clathrin accumulation preceded the actin rearrangements necessary for Listeria entry. These data provide evidence fora clathrin-based entry pathway allowing internalization of large objects (bacteria and ligand-coated beads) and used by zippering bacteria as part of a general mechanism to invade host mammalian cells. We also revealed a nonendocytic role for clathrin required for extracellular EPEC infections.
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