Time course of hepatic gene expression and plasma vitellogenin protein concentrations in estrone-exposed juvenile rainbow trout (Oncorhynchus mykiss)
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| Authors: | Osachoff, HL; Brown, LLY; Tirrul, L; van Aggelen, GC; Brinkman, FSL; Kennedy, CJ |
| Year: | 2016 |
| Journal: | Comparative Biochemistry and Physiology D-Genomics & Proteomics 19: 112-119 Article Link (DOI) |
| Title: | Time course of hepatic gene expression and plasma vitellogenin protein concentrations in estrone-exposed juvenile rainbow trout (Oncorhynchus mykiss) |
| Abstract: | Estrone (E1), a natural estrogen hormone found in sewage effluents and surface waters, has known endocrine disrupting effects in fish, thus, it is a contaminant of emerging concern. Juvenile rainbow trout (Oncorhynchus mykiss) were exposed to an environmentally-relevant concentration of E1 (24 ng/L E1 [0.1 nM]) for 7 d and then placed in clean water for a 9 d recovery period. RNA sequencing showed transcripts from numerous affected biological processes (e.g. immune, metabolic, apoptosis, clotting, and endocrine) were altered by E1 after 4 d of treatment. The time course of E1-inducible responses relating to vitellogenesis was examined daily during the two phases of exposure. Hepatic gene expression alterations evaluated by quantitative polymerase chain reaction (QPCR) were found during the treatment period for vitellogenin (VTG), vitelline envelope proteins (VEPs) alpha, beta and gamma, and estrogen receptor alpha 1 (ER alpha 1) transcripts. ER alpha 1 was the only transcript induced each day during the treatment phase, thus it was a good indicator of E1 exposure. Gradual increases occurred in VEP beta and VEP gamma transcripts, peaking at d7. VTG transcript was only elevated at d4, making it less sensitive than VEPs to this low-level E1 treatment. Inductions of ER alpha 1, VEP alpha, VEP beta and VEP gamma transcripts ceased 1 d into the recovery phase. Plasma VTG protein concentrations were not immediately elevated but peaked 7 d into the recovery phase. Thus, elevated vitellogenesis-related gene expression and protein production occurred slowly but steadily at this concentration of E1, confirming the sequence of events for transcripts and VTG protein responses to xenoestrogen exposure. (C) 2016 Published by Elsevier Inc. |
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